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CH Diagnostic & Consulting Service, Inc. 512 5th Street Berthoud, CO 80513
email: gsturbaum@chdiagnostic.com website: www.chdiagnostic.com
EDUCATION 1999 - 2003: University of Arizona, Tucson, AZ 85721 Doctor of Philosophy: Pathobiology 1995 - 1999: Colorado State University, Ft. Collins, CO 80523 Master of Science: Microbiology 1988 - 1993: Colorado State University, Ft. Collins, CO 80523 Bachelors of Science: Microbiology Bachelors of Science: Biochemistry RESEARCH EXPERIENCE 1999 - 2003: Ph.D. Research with Dr. Charles R. Sterling. University of Arizona. Molecular analysis of Cryptosporidium parvum related to issues of molecular detection and host specificity/speciation issues. 1995 - 1999: Master's Research with Dr. Donald A. Klein. Colorado State University. Utilizing Polymerase Chain Reaction (PCR) and Single Stranded Conformation Polymorphism (SSCP) for identifying variability in the internal transcribed region (ITS-1) - rDNA of Cyclospora cayetanensis; phylum: Apicomplexa. 1998: Visiting Researcher. Lima, Peru, John Hopkins University, and University of Arizona. A six-month sabbatical confirming the detection of Cyclospora cayetanensis in wastewater by classical and molecular techniques while in Lima, Peru. 1995 - 1997: Research Associate. Colorado State University. Biological Particle Surrogates for Filtration Performance Evaluation; Project 181; American Water Association Research Foundation. Research associate overseeing growth, seeding, and enumeration of bacteria, bacteria-phages, and protozoans, including Giardia lamblia and Cryptosporidium parvum. 1992 - 1993: Laboratory Assistant, Dr. Whalen. Colorado State University. Duties included care and passage of fibroblast cells, performing reverse transcriptase assays, and FeLV antigen ELISA. PROFESSIONAL EXPERIENCE 2001 - Present: Owner and President, CH Diagnostic & Consulting Service, Inc. Berthoud, CO 80513. 1997 - 1999: Laboratory Director. CH Diagnostic & Consulting Services, Inc. Loveland, CO 80537. Provided direction of day-to-day operation of laboratory for all samples and technicians/analysts. Involved in implementing and training of new and standard operating procedure protocols. 1993 - 1997: Laboratory Analyst. CH Diagnostic & Consulting Services, INC. Loveland, CO 80537. Conducted sample processing and microscopic analyses. Approved Analyst: Method 1623 (Immunomagnetic isolation of Cryptosporidium oocysts and Giardia cysts water from water samples). 2001, USEPA, EPA-821-R-01-026. Approved Analyst: ICR (Information Collection Rule). ICR Laboratory Manual, 1996, USEPA, EPA-600-R95-178. RESEARCH INTERESTS Development of highly sensitive and specific PCR-based methodologies related to detecting of waterborne pathogens, including, Cryptosporidium spp., and Giardia spp. FUNDED RESEARCH PROJECTS AwwARF Project 4099, Development of a Cryptosporidium Speciation Protocol for Source Water Protection. 2008. DiGiovanni, GD, Hoffman, B, Sturbaum GD. American Water Works Research Foundation (AwwaRF), Denver Colorado. AwwARF Project 2901, Molecular Methods for Microsporidia Detection (MMMD). 2005. Wolk DM, Sterling CR, Marshall MM, Sturbaum GD. American Water Works Research Foundation (AwwaRF), Denver Colorado. Environmental Protection Agency. 2002. Evaluation of PCR detection with low, known numbers of Cryptosporidium parvum oocysts. USEPA. PUBLISHED MANUSCRIPTS Sturbaum GD, DA Schaefer, BH Jost, CR Sterling, MW Riggs. 2008. Antigenic differences within the Cryptosporidium hominis and Cryptosporidium parvum surface proteins P23 and GP900 defined by monoclonal antibody reactivity. Molecular and Biochemical Parasitology. June, 159(2):138-141. Wolk, DM, GD Sturbaum, R Hoffman. 2007. Molecular Methods for Microsporidia Detection: Use of an Inhibitor Control with Real-Time PCR. Sponsored by AwwARF Project 2901. Published by AwwARF, AWWA and IWA Publishing. Hendricks, DW, WF Clunie, GD Sturbaum, DA Klein, TL Champlin, P Kugrens, J Hirsch, B McCourt, GR Norby, MD Sobsey, DJ Hunt, MJ Allen. 2005. Filtration Removals of Microorganisms and Particulates. Journal of Environmental Engineering. Dec. 131(12): 1621-1632. Sturbaum GD, BH Jost, CR Sterling. 2003. Nucleotide changes within three Cryptosporidium parvum surface protein encoding genes differentiate genotype I from genotype II isolates. Molecular and Biochemical Parasitology. Apr 25; 128(1): 87-90. Sturbaum, GD, PT Klonicki, MM Marshall, BH Jost, BL Clay, and CR Sterling. 2002. Immunomagnetic separation (IMS)-fluorescent antibody detection and IMS-PCR detection of seeded Cryptosporidium parvum oocysts in natural waters and their limitations. Applied and Environmental Microbiology. 68(6): 2991-6. Reed, CM, GD Sturbaum, PJ Hoover, and CR Sterling. 2002. Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis. Applied and Environmental Microbiology. 68(1): 427-9. Sturbaum, GD, C Reed, PJ Hoover, BH Jost, MM Marshall, and CR Sterling. 2001. Species-Specific, Nested PCR-Restriction Fragment Length Polymorphism Detection of Single Cryptosporidium parvum Oocysts. Applied and Environmental Microbiology. 67:2665-2668. Sturbaum, GD, YR Ortega, RH Gilman, CR Sterling, L Cabrera, and DA Klein. 1998. Detection of Cyclospora cayetanensis in Wastewater. Applied and Environmental Microbiology. 64(6): 2284-2286. Klonicki PT, CM Hancock, TM Straub, SI Harris, KW Hancock, AN Alyaseri, CM Meyer and GD Sturbaum. 1997. Crypto research: are fundamental data missing? Jour. AWWA, 89(9): 97-103. Hancock CM, JV Ward, KW Hancock, PT Klonicki and GD Sturbaum. 1996. Assessing plant performance using MPA. Jour. AWWA, Dec. 1996. Anderson WL, TL Champlin, WF Clunie, DW Hendricks, DA Klein, P Kugrens and GD Sturbaum. 1996. Biological Particle Surrogates for Filtration Performance Evaluation. Proceedings of the AWWA 1996 Water Technology Conference, Toronto, Ontario, 1996. PUBLISHED BOOK CHAPTERS Sturbaum, GD, G Di Giovanni. 2006. Waterborne Parasites and Diagnostic Tools in Foodborne Parasites. Pages 231 - 255. Editor: Y Ortega. Kluwer Academic Publishers. Springer Science+Business Media, LLC. Contributor to the USEPA publication Quality Assurance/Quality Control Guidance for Laboratories Performing PCR Analyses on Environmental Samples (EPA 815-B-04-001; 2004) PRESENTATIONS and WORKSHOPS Instructor, 2008. Microbiology. Leadville Operator School; Beginning Water Course. Leadville, Colorado, To Be Determined, 2008. Instructor, 2008. Microbiology. Boulder Operator School; Intermediate Water Course. Boulder, Colorado, March 24-28, 2008. Workshop Organizer and Presenter: How to Implement a PCR Based Method in an Environmental Laboratory. Water Quality Technology Conference (WQTC). November 4-8, 2007, Charlotte, North Carolina. Instructor, 2007. Microbiology. Leadville Operator School; Beginning Water Course. Leadville, Colorado, June 25-27, 2007. Invited Speaker. Review of Cryptosporidium spp. and Giardia lamblia life cycles, health impact, and the EPA Method 1623 for detecting oocysts and cysts in water. Rocky Mountain Water Quality Analysts Association. 2007 Annual Symposium Speaker Form. Friday, May 11th, 2007 Instructor, 2007. Microbiology. Boulder Operator School; Intermediate Water Course. Boulder, Colorado, March 26-30, 2007 Workshop Organizer: Ecological Interactions of Algae in Water Supplies. Water Quality Technology Conference (WQTC). November 5 - 9, 2006, Denver, Colorado. Instructor, 2006. Microbiology. Leadville Operator School; Beginning Water Course. Leadville, Colorado, June 25-27, 2006 Instructor, 2006. Microbiology. Boulder Operator School; Intermediate Water Course. Boulder, Colorado, March 21-25, 2006 Instructor, 2005. Microbiology. Leadville Operator School; Beginning Water Course. Leadville, Colorado, June 24-28, 2005 Instructor, 2005. Microbiology. Boulder Operator School; Intermediate Water Course. Boulder, Colorado, March 27-31, 2005 Invited Speaker. Molecular detection methods for parasitic infections. Food Safety: Parasites in meats and fresh produce. University of Georgia Griffin Experiment Station. Griffin, Georgia. July 10-11, 2003. Workshop Organizer and Presenter: Molecular Detection Techniques: Current Status and Limitations. Water Quality Technology Conference (WQTC). November 14-18, 2004, San Antonio, Texas. Invited Speaker. Molecular detection methods for parasitic infections. Food Safety: Parasites in meats and fresh produce. University of Georgia Griffin Experiment Station. Griffin, Georgia. July 24-26, 2002. Workshop Organizer and Presenter: Advanced Techniques for Detecting Waterborne Contaminants. Water Quality Technology Conference (WQTC). November 10-14, 2002, Seattle, Washington. Invited Speaker. Molecular detection methods for parasitic infections. Food Safety: Parasites in meats and fresh produce. University of Georgia Griffin Experiment Station. Griffin, Georgia. July 24-26, 2001. Presentation: Evaluation of nested-PCR detection rates with known low number of Cryptosporidium parvum oocysts. Microbial Molecular Methods Workshop 101. Water Quality Technology Conference (WQTC). November 11-15, 2001, Nashville, Tennessee. Workshop Organizer and Presenter: Detection and Identification of Eukaryotic Intestinal Parasites by Molecular Techniques: Pro’s and Con’s. Water Quality Technology Conference (WQTC). November 11-15, 2001, Nashville, Tennessee. POSTER PRESENTATIONS Sturbaum, GD, WC Black IV, DA Klein, and CR Sterling. Differences observed within the internal transcribed spacer (ITS-1) of Cyclospora cayetanensis by single stranded conformation polymorphism (SSCP) and direct sequencing: Geographic source relationships and implications. American Society of Tropical Medicine and Hygiene. Houston, Texas. 2000. PROFESSIONAL MEMBERSHIPS American Water Works Association Vice Chair, Organisms in Water, American Water Works Association
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