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Method 1623
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Cryptosporidium and Giardia Analysis via Method 1623

This USEPA approved method entails filtration, immunomagnetic separation, and immunofluorescent microscopy, and is used primarily for the detection of Cryptosporidium and Giardia in raw water sources. In addition, this method involves the application of DAPI (a DNA stain) and the use of differential interference contrast (DIC) microscopy to aid in the confirmation of oocysts and cysts. Currently, multiple filter types are approved for use in sample concentration including the Pall HV Envirochek™ capsule and the IDEXX Filta-Max™ module. At CH Diagnostic, we solely process samples with the Gelman HV Envirochek™ capsule. 

            10L Grab Sampling Instructions (pdf)

    Filter-in-the-Field Sampling Instructions (pdf)

    Analysis Request Form (pdf)

Per Method 1623 QA/QC stipulations, matrix spike (MS) analyses are required to estimate Giardia and Cryptosporidium recovery efficiencies from different water sources. Method 1623 prescribes an MS analysis on all raw waters beginning with the first sample and then every 20th sample thereafter.



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Last modified: June 11, 2007